The Mouse Phenotyping Core is organized around the three services Body Composition, Whole Body Metabolic Assessment and Metabolic Clamp.
Body Composition measurements can be made through two systems that the Mouse Phenotyping Core operate: Dual energy x-ray absorbance (DEXA) and more recently added nuclear magnetic resonance imagining (MRI) analysis. The DEXA scanner (Piximus2) machine is located outside the mouse barrier facility and is most often used for terminal measurements or when bone density measurements are required. The MRI is located within the Berrie barrie facility and is most useful for serial measurements in mice without anesthesia. Scheduling an experiment with the core staff is done directly.
The Whole Body Metabolic Assessment program allows researchers to measure whole body mouse’s metabolic activity. Through the system, the mouse’s energy expenditure, food and water intake, movement, CO2 expenditure and RQ/RER are continuously measured, in up to sixteen mice per run. The system is located within a dedicated set of rooms within the Berrie Mouse Barrier Facility. Scheduling an experiment is done with the core staff directly.
Measurement of glucose utilization and insulin action are performed through Metabolic Clamp program. This system permits investigators to assess insulin sensitivity through basal and hyperinsulinemic euglycemic clamps studies in conscious mice. Tracer studies permit assessment of glucose uptake by individual tissues and estimation of hepatic gluconeogenesis. The analysis is performed within the Berrie Center.
In the next six months we plan to begin an Enteral Feeding Program. Placement of permanent catheters directly into the gasterointestinal tract, investigators will be able to manipulate food intake directly. This will be especially helpful to investigators interested in overfeeding and pair feeding studies, and in the manipulation of the gut microbiome.
|DEXA (Piximus2)||MRI (Bruker)||Calorimetry
|Mouse Clamp||Survival surgery||• Bone density (g/cm2)
• Lean mass (g)
• Fat mass (g)
• Mouse body width and length (cm)
|• Lean mass (g)
• Fat mass (g)
• Fluid (g)
In living mice
• dO2/dCO2- differences in O2 and CO2 concentration values
• VO2/VCO2 – O2 consumption and CO2 production
• RER – respiratory exchange rate
• H – heat production in kcal/h/kg
• Drink/Feed – consumption in ml or g
• Running Wheel –treadmill for exercise
|• Hyperinsulinemic euglycemic clamp – insulin action for whole body and tissue specific.
• Hyperglycemic clamp – insulin secretion and beta cell function.
|• Jugular vein and carotid artery cannulation.
• Catheterization of gastero-intestinal tract for over feeding study.
Anthony W. Ferrante, Jr., MD, PhD : Director, Mouse Phenotyping Core, Russ Berrie Pavilion, Room 238, 1150 St.Nicholas Avenue, New York, NY 10032. Phone: 212-851-5322