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Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans.

Citation
Meng, J., et al. “Global Profiling Of Distinct Cysteine Redox Forms Reveals Wide-Ranging Redox Regulation In C. Elegans.”. Nature Communications, p. 1415.
Center Joslin Diabetes Center
Author Jin Meng, Ling Fu, Keke Liu, Caiping Tian, Ziyun Wu, Youngeun Jung, Renan B Ferreira, Kate S Carroll, Keith Blackwell, Jing Yang
Abstract

Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after HO treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology.

Year of Publication
2021
Journal
Nature communications
Volume
12
Issue
1
Number of Pages
1415
Date Published
12/2021
ISSN Number
2041-1723
DOI
10.1038/s41467-021-21686-3
Alternate Journal
Nat Commun
PMID
33658510
PMCID
PMC7930113
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