Columbia Translational Biomarker Analytical Core

Overview

The Translational Biomarker Analytical Core (TBAC) makes available to Diabetes Research Center investigators a variety of high quality radioimmunoassay and other analyses that are critical to basic, translational and clinical investigators studying diabetes mellitus, insulin resistance, insulin and C-peptide secretion, glucagon dynamics, energy homeostasis, and complications of diabetes.

The services provided are best based in a centralized core laboratory because they require special instrumentation and methods that are difficult or impracticably expensive to establish in the laboratories of individual members of the research base. This arrangement also allows for coordinated consultations and improved reliability and reproducibility of the various assays employed. In addition to high quality radio-immunoassay, ELISA, and other analytical methods, TBAC facilitates access to the measurement of small molecules by targeted metabolomics/lipidomics at the Irving Institute’s Metabolomics Core.

Services

1. Consulting services for study design, sample processing, assay methods for diabetes clinical investigators
2. Large variety of RIAs: GLP-1, PYY, oxyntomodulin, ghrelin, C-peptide, insulin, pro-insulin, glucagon, leptin, adiponectin, etc.
3. Large variety of ELISA: GLP-1 total and active, GIP, acetaminophen, orexin, cortisol, Substance amyloid A, cytokines, etc.
4. Lipids, glucose, glycerol, FFA, nitrogen as well as bomb calorimetry analysis
5. Rapid 1-2 months turnover for most assays
6. Assays can be developed on demand, based on investigator needs